[PMC free article] [PubMed] [Google Scholar] 19. The presence of anti\RBD antibodies was recognized using Access SARS\CoV\2 IgM and IgG II antibody checks, while nAbs were measured by sVNT. The level of sensitivity and specificity of sVNT were 94.4% and 98.9%, respectively. There were strong positive correlations between the inhibition ideals of sVNT and the results of the Access SARS\CoV\2 IgM (or median and IQR and were compared using the test and the Wilcoxon rank\sum test for VU0652835 VU0652835 parametric and nonparametric data, respectively. Linear regression analysis was used to assess the relationship between each continuous variable. Rho ideals were analyzed from the Spearman rank\order correlation coefficient. A two\sided value <0.05 was considered statistically significant. All statistical analyses were carried out using R (v4.0.2; R Basis for Statistical Computing, Vienna, Austria; http://www.R-project.org/). 3.?RESULTS 3.1. Level of sensitivity and specificity of the access IgM and IgG antibody assays for COVID\19 Distributions of S/CO and AU/ml for the Access IgM and IgG II assays for COVID\19 and non\COVID\19 sera are demonstrated in Number?1A,B, respectively. In the IgM assay, the median S/CO value of the COVID\19 samples was 16.8 (IQR: 7.0C37.8), while that of the non\COVID\19 samples was 0.2 (IQR: 0.2C0.2) (p?p?
IgM antibody assayCut\off S/CO?1.089089100%5452296.3%(95.9C100)(87.3C99.5)IgG II antibody assayCut\off IgG 10?AU/ml8918898.9%5451394.4%(93.9C99.9)(84.6C98.8)sVNTCut\off inhibition 20%8968393.3%5451394.4%(85.9C97.5)(84.6C98.8)Cut\off inhibition 30%8918898.9%5451394.4%(93.9C99.9)(84.6C98.8) Open in a separate windows Abbreviations: AU, arbitrary models; CI, confidence interval; COVID\19, coronavirus disease 2019; IgG, immunoglobulin G; IgM, immunoglobulin M; nAb, neutralization antibody; S/CO, transmission for the test sample/transmission at slice\off value; sVNT, surrogate computer virus neutralization test. 3.2. Alternate cut\off point, sensitivity, and specificity for sVNT The alternative cut\off value for sVNT was decided using Rabbit Polyclonal to CDCA7 serum samples from COVID\19 patients (n?=?54) and non\COVID\19 patients (n?=?89). Median inhibition was 91.4% (IQR: 84.6%C94.8%) for COVID\19 and 5.9% (IQR: 0%C12.1%) for non\COVID\19 in sVNT (p?