Photos were taken having a camera mounted on a light microscope (Axioplan, Zeiss). Apoptosis assay We induced apoptosis in LCLs (5*105 cell per very well of the 48-well-plate) transformed by All or crazy type infections at 40C60 dpi with the addition of Etoposide (4g/ml, Sigma Aldrich) or Staurosporine (4g/ml, Sigma-Aldrich) towards the tradition moderate for…
Category: Vesicular Monoamine Transporters
This screen was conducted with two different people using one cytometer at a rate of 24 plates per day including analysis and took 26 days to screen the 195,000 compounds in the library at that time
This screen was conducted with two different people using one cytometer at a rate of 24 plates per day including analysis and took 26 days to screen the 195,000 compounds in the library at that time. on each bead set is easily obtained. An arbitrary cutoff of 40% inhibition was taken to indicate a screening…
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2). a synthesis inhibitor), HgCl2, and AgNO3. DoseCresponse curves for the decrease in TR following exposure to each inhibitor were developed. Decreases in TR of N01-11136 following treatment with inhibitors were up to 60% for CHX, 82% for HgCl2, and 42% for AgNO3. These results indicate that this symplastic pathway terminating in the guard cells…